Insilico primer designing of structural region of Dengue virus for molecular diagnostic

Dengue has reemerged significantly from the past two decades for the public health problems. Clinicians require a diagnostic test with high sensitivity, which is applicable during early symptomatic phase. PCR is a specific and sensitive molecular technique to detect the gene for monitoring possible Dengue outbreaks and circulating viral serotypes. Bioinformatics tools were used to design and verify the novel Dengue subtype specific primers. In this investigation, 24 primers targeting the structural region of four variants of dengue virus were designed through in silico. There is no cross reactivity found with the primers designed with any other virus. All these sets of primers of dengue were highly specific and may perhaps be helpful for the molecular diagnostic of Dengue viruses.